Applications of Single-Molecule Methods to Membrane Protein Folding Studies

Exploring protein folding landscapes: from ensembles to single molecule methods

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Single-molecule fluorescence studies of Protein Folding and Molecular Chaperones

Folding of newly synthesized proteins is an essential part of protein biosynthesis and misfolding can result in protein aggregation which can also lead to several severe diseases. Protein folding is a highly heterogeneous process and rarely populated intermediate states may play an important role. Single-molecule techniques are ideally suited to resolve these heterogeneities. In this thesis, I ...

Single-molecule fluorescence studies of protein folding and conformational dynamics.

Single-molecule measurement of protein folding kinetics.

In order to investigate the behavior of single molecules under conditions far from equilibrium, we have coupled a microfabricated laminar-flow mixer to a confocal optical system. This combination enables time-resolved measurement of Förster resonance energy transfer after an abrupt change in solution conditions. Observations of a small protein show the evolution of the intramolecular distance d...

Single Molecule Fluorescence Investigation of Protein Folding

Title of Document: SINGLE MOLECULE FLUORESCENCE INVESTIGATION OF PROTEIN FOLDING Jianwei Liu, Ph.D., 2008 Directed By: Associate Professor, Victor Muñoz, Department of Chemistry and Biochemistry In addition to the well known two-state folding scenario, the energy landscape theory of protein folding predicts the possibility of downhill folding under native conditions. This intriguing prediction ...